Deadline for applications: 30 March 2025
Starting date: June or September 2025
Duration: 1 year, with possible extension
Starting date: June or September 2025
Duration: 1 year, with possible extension
We are looking for a research scientist/engineer to spearhead the development of new transgenic markers and tools in the crustacean Parhyale.
Background – Our team studies leg regeneration in the crustacean Parhyale hawaiensis using transgenic tools, live imaging and molecular profiling approaches. We have already established a range of transgenic markers and tools in this species (see Averof 2022, Paris et al. 2022). We rely on transgenic approaches to identify and to study cis-regulatory elements, to develop transgenic reporters for live imaging, and to manipulate gene expression in a conditional and cell-type-specific manner. These efforts require generating transgenic lines efficiently and on a large scale. The recruited developer/engineer will spearhead this effort, both as a manager and a hands-on experimenter.
Activities – The recruited person will become familiar with the microinjection and transgenesis methods that have already been established in Parhyale. S/he will then coordinate the transgenic activities of the team, including streamlining procedures (microinjection, screening of transformants, establishing lines), developing new tools and transgenic lines of broad interest (e.g. cell/tissue-specific markers, tools for conditional gene expression, mosaic labelling and cell ablation), and training new team member in these approaches. S/he will author publications on the methods and tools s/he develops and the projects to which s/he contributes.
Activities – The recruited person will become familiar with the microinjection and transgenesis methods that have already been established in Parhyale. S/he will then coordinate the transgenic activities of the team, including streamlining procedures (microinjection, screening of transformants, establishing lines), developing new tools and transgenic lines of broad interest (e.g. cell/tissue-specific markers, tools for conditional gene expression, mosaic labelling and cell ablation), and training new team member in these approaches. S/he will author publications on the methods and tools s/he develops and the projects to which s/he contributes.
Work environment – The Institute of Functional Genomics of Lyon (IGFL) is an internationally renowned research centre. Scientific innovation and interdisciplinary collaborations are at the heart of our activities. The transgenic tools developer/engineer will be integrated in the Averof lab (www.averof-lab.org), where s/he will play a key role in establishing transgenic tools, in close collaboration with the team leader, postdocs, doctoral students and technicians. He/she will participate in the team's meetings, seminars, lab retreats and training. The team’s working language is English.
The post is initially funded for one year by a grant from the Fondation pour la Recherche Médicale, with a possible extension depending on additional funding. The envisaged starting date could be in June or September 2025.
The post is initially funded for one year by a grant from the Fondation pour la Recherche Médicale, with a possible extension depending on additional funding. The envisaged starting date could be in June or September 2025.
Expected qualifications
* Experience in microinjection, transgenesis and/or CRISPR
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English
* Experience in microinjection, transgenesis and/or CRISPR
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English