Deadline for applications: 29 March 2025
Starting date: June or September 2025
Duration: 1 year, with possible extension
Starting date: June or September 2025
Duration: 1 year, with possible extension
We are looking for a research scientist/engineer to spearhead the development of new transgenic markers and tools in the crustacean Parhyale.
Background – Our team studies leg regeneration in the crustacean Parhyale hawaiensis using transgenic tools, live imaging and molecular profiling approaches (see Averof 2022, Paris et al. 2022).
We have already established a range of transgenic markers and tools in this species (summarized in https://tinyurl.com/NatMeth, and reviewed in https://tinyurl.com/ParhyaleRev). We rely on transgenic approaches to identify and to study cis-regulatory elements, to develop transgenic reporters for live imaging, and to manipulate gene expression in a conditional and cell-type-specific manner. These efforts require generating transgenic lines efficiently and on a large scale. The recruited developer/engineer will spearhead this effort, both as a manager and a hands-on experimenter.
Activities – The recruited person will become familiar with the microinjection and transgenesis methods that have already been established in Parhyale. S/he will then establish an efficient transgenesis pipeline, including streamlined procedures for microinjection, screening for transformants, and establishing transgenic lines. S/he will manage the transgenesis infrastructure and collaborate closely with other team members to help them implement their transgenesis experiments. In parallel to supporting team projects, the developer/engineer will have opportunities to develop his/her own projects, testing new tools and generating transgenic lines of broad interest (e.g. cell/tissue-specific markers, Brainbow-like tools for mosaic labelling, tools for conditional cell ablation). S/he will author publications on the methods s/he develops and the projects to which s/he contributes.
Work environment – The transgenic tools developer/engineer will be fully integrated in the Averof lab (averof-lab.org), participating in lab meetings, seminars, lab retreats and training. S/he will collaborate closely with the team leader and other team members (postdocs, PhD students, technicians) to provide support, discuss and prioritise objectives. The lab’s working language is English.
The post is funded initially for one year, by a grant from the Fondation pour la Recherche Médicale, with a possible extension depending on additional funding.
We have already established a range of transgenic markers and tools in this species (summarized in https://tinyurl.com/NatMeth, and reviewed in https://tinyurl.com/ParhyaleRev). We rely on transgenic approaches to identify and to study cis-regulatory elements, to develop transgenic reporters for live imaging, and to manipulate gene expression in a conditional and cell-type-specific manner. These efforts require generating transgenic lines efficiently and on a large scale. The recruited developer/engineer will spearhead this effort, both as a manager and a hands-on experimenter.
Activities – The recruited person will become familiar with the microinjection and transgenesis methods that have already been established in Parhyale. S/he will then establish an efficient transgenesis pipeline, including streamlined procedures for microinjection, screening for transformants, and establishing transgenic lines. S/he will manage the transgenesis infrastructure and collaborate closely with other team members to help them implement their transgenesis experiments. In parallel to supporting team projects, the developer/engineer will have opportunities to develop his/her own projects, testing new tools and generating transgenic lines of broad interest (e.g. cell/tissue-specific markers, Brainbow-like tools for mosaic labelling, tools for conditional cell ablation). S/he will author publications on the methods s/he develops and the projects to which s/he contributes.
Work environment – The transgenic tools developer/engineer will be fully integrated in the Averof lab (averof-lab.org), participating in lab meetings, seminars, lab retreats and training. S/he will collaborate closely with the team leader and other team members (postdocs, PhD students, technicians) to provide support, discuss and prioritise objectives. The lab’s working language is English.
The post is funded initially for one year, by a grant from the Fondation pour la Recherche Médicale, with a possible extension depending on additional funding.
Expected qualifications –
* Experience in microinjection, transgenesis and/or CRISPR
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English
* Experience in microinjection, transgenesis and/or CRISPR
* Experience in cell or developmental biology would be an advantage, but not essential
* Organisational and interpersonal/collaborative skills
* Communication in English